Food Standards (Application A1172 – Enzymatic Production of Rebaudioside D) Variation

The Board of Food Standards Australia New Zealand gives notice of the making of this variation under section 92 of the Food Standards Australia New Zealand Act 1991. The variation commences on the date specified in clause 3 of this variation.

Dated 28 August 2019

$C:\Users\coughc\AppData\Local\Microsoft\Windows\INetCache\Content.Word\Esig.png$

Joanna Richards

Standards Management Officer

Delegate of the Board of Food Standards Australia New Zealand

Note:

This variation will be published in the Commonwealth of Australia Gazette No. FSC 128 on 5 September 2019. This means that this date is the gazettal date for the purposes of clause 3 of the variation.

1 Name

This instrument is the Food Standards (Application A1172 – Enzymatic Production of Rebaudioside D) Variation.

2 Variation to standards in the Australia New Zealand Food Standards Code

The Schedule varies Standards in the Australia New Zealand Food Standards Code.

3 Commencement

The variation commences on the date of gazettal.

Schedule

[1] Schedule 3 is varied by omitting paragraph S3—35(2)(b), substituting

(b) by enzymatic conversion of purified stevia leaf extract to produce rebaudioside M using protein engineered enzymes that:

(i) contain both UDP‑glucosyltransferase (EC 2.4.1.17) and sucrose synthase (EC 2.4.1.13) components; and

(ii) are sourced from both of the following:

(a) a Pichia pastoris strain expressing UGT-A;

(b) a Pichia pastoris strain expressing both UGT-B1 and UGT-B2;

(c) by enzymatic conversion of purified stevia leaf extract to produce rebaudioside D using a protein engineered enzyme that:

(i) contains both UDP‑glucosyltransferase (EC 2.4.1.17) and sucrose synthase (EC 2.4.1.13) components; and

(ii) is sourced from Pichia pastoris strain UGT-A.

[2] Schedule 18 is varied by inserting in the table to subsection S18—9(3), in alphabetical order

Protein engineered enzyme that: contains both UDP‑glucosyltransferase (EC 2.4.1.17) and sucrose synthase (EC 2.4.1.13) components; and is sourced from Pichia pastoris strain UGT-A.	For the conversion of purified stevia leaf extract to produce rebaudioside D.	GMP